Sequence complexity of circular Epstein-Bar virus DNA in transformed cells
نویسندگان
چکیده
منابع مشابه
Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells.
A high-density fraction of high-molecular-weight DNA was isolated from the human lymphoid cell line Raji. This cell line contains 50 to 60 virus genome equivalents of Epstein-Barr virus DNA per cell, and the high-density DNA fraction was 10-fold enriched in such viral sequences. Sedimentation analysis on neutral glycerol gradients, followed by hybridization experiments with viral complementary ...
متن کاملViral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.
A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as 5 hr, and required ATP, deoxynucleoside triphosphates, magnesium, and a calcium chelator. In nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the ...
متن کاملTHE IN VITRO GROWTH PROPERTIES OF CELL LINES FROM EPSTEIN-BARR VIRUS-INDUCED TAMARIN TUMORS AND TAMARIN B CELLS TR ANSFORMED BY EPSTEIN BARR VIRUS
EBV-carrying human cell lines, depending on whether the cells are derived from Burkitt's lymphoma (BL) tumor biopsies or transformed by EBV in vitro, have different growth properties in vitro. In contrast, there are no clear differences between tamarin tumor lines and tamarin LCLs in vitro. Both types of tamarin cell lines could grow in agarose and formed colonies unlike human LCLs, althoug...
متن کاملCharacterization of the transcriptional repressor RBP in Epstein-Barr virus-transformed B cells.
RBP, a transcriptional repressor, is intricately involved in Epstein-Barr virus (EBV) transformation of human B cells. The EBV nuclear proteins EBNA-2, -3, -4 and -6 all utilize RBP to regulate the transcription of both cellular and viral genes. This study investigates the isoforms of the RBP protein in Burkitt's lymphoma (BL) cells and in EBV-transformed lymphoblastoid cell lines (LCLs). Two-d...
متن کاملRelease of infectious Epstein-Barr virus by transformed marmoset leukocytes.
Marmoset blood leukocytes transformed in vitro by Epstein-Barr virus regularly release extracellular infectious Epstein-Barr virus with high titers of transforming activity. By comparison, human umbilical cord leukocytes and adult human leukocytes transformed by Epstein-Barr virus release either no extracellular infectious virus or small amounts, irregularly.
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1981
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.40.1.11-19.1981